WHEAT (Triticum Aestivum L.) is an imported ceerreal crop, Contributing 20% of the daily call intake, is an rich source and carbohydrates in the human Diet. Grain yield is the most Important eConomic Parameter and is influenced by Several Biotic andAbiotic Factors. The Demand for WHEAT is Forecast to Increase by 50% by 2050 (). To Cope with Abiotic Challenges Such As Salinity, Drough, and Heat, Plants Employ a variety of Stress-Responsive Pathways and Activated Defense Mechaanisms1. Globally, TheSeENVIRONMENTAL CONDRAINTS Lead to Average Production Losses of more than 50% in Main Crops2. To Meet the Food Demands of the Growing Human Populating, Breeding ToLerant Varieties Resistant to various Environmental Challenges, as well as IMPROVED Quality and Yield3 Are Future GOALS. SINCETraDitional Breeding has had little little Success in Abiotic Stress Due to iTS Multigenic Narrow Genetic Pool. -tolerant Cultivars is a viable option. For the development of stress-timent plants, understanding the molecular mechanisms and explororing the stress-Responsive generals that regulating plants to Abiotic Stresses4, 5. TIC Stress. TheReface, Identifying Relevant TFS is Stream for Studying Signaling Pathways and Abiotic Stress.Strategy to Identify Wheat Genes.
After the First Three Functionally RECOGNIZED MEMBERS, Gibberllic Acid Insensitive (GAI), A REPressor of Gai (RGA), and A ScareCrow (SCR), Gras, A Key TF Gene Family in Plants, WAS VERIFIED6,7,8. The Carboxyl Terminusof Gras Proteins The Highly Conserved Sequences Lhr I, VHIID, LHR II, PFyre, Which Range from 400 TO 770 AMINO Acids9,10,11. Ved Motifs Play An Important Role in Protein Function12, 13. ForExample, in Arabidopsis, Mutations of the PFyre and Saw Motifs in the RGA and Slr1 Regions Cause Phenotypic AlTerations8, 12. Gras Proteins Have More Amino-Termini in Leength and Sequence, Impoling Functions'Been Explored in Several Plant Species11. Initially, BASED on Shared Features and Sequence Similalarities, Eight Subfamilies of the Gras Gene Family FIED: SCL3, SCR, SHR, LISCL, PAT1, DELLA, and HAM. Each Subfamily May Have a UNIQUEFunction in a Plant's Physiology Activities11. For Example, The Arabidopsis Scr gene, Which Governs the Radial for the Root, was the first Gras Er discovered6. Through the scr/shr complex, arabidopsis shr, a member of the gras gene family, isAlso Necessary for Root Development13,14,15. Atscl3 (scarecrow-like 3) Has Been DEMONSTRATED to Positive Control the Gibberllin (GA) Pathway and Operate as a La Repressor, Regulating Ga Homeostasis in Root Development. In Composition Studies of their Regulatory Mechanisms, Della Proteins Wee Revealed to Be Repressors of Ga Signaling8. The Members of the Della Subfamily Gai, RGL1 (RGA-LIKE1), RGL2, and RGL3 Can be distinguish ED FROM OTHER GRAS Proteins by the Conserved Della Motif in Their N-TerminalRegion. The Pat1 Subfamily Member Linked to Atscl13 is Largly A Positive Regulator of Phytochrome B (PHYB) Signaling, in Contract to Others of This Subfami. ly, such as pat1 and scl21, which are involved in Phytochrome a (PHYA) Signaling. Rice Monoculm 1 (OSMOC1) Expression in Axillary Buds is Critical for Regulating Rice Tilleric S OSMOC1-Like Genes that are also important for the control of axillar meristem outGropGras Transcript Factor from Brassica Rapa L., is involved in Drought Stress TOLARANCE in Transgenic Arabidopsis18. Several Gras Members, Including NSP1 AN D NSP2 (or their Protein Complex), Are Required for Noduering in Medicago Truncatula Gaertn. As Putative Regulators of Nod-Factor-inducible Gene Expression19, 20. FURTHERMORE, NSP1 and NSP2 Are Necessary for Strigolactone Production in M. Truncatula and Overza Sativa L.5. A Recent Study Has sh Own that the gras transcripting factors SCL6/SCL6-IV, SCL22/SCL6-III,And SCL27/SCL6-II Are Regulated By MiRNA171 in Arabidopsis21,22,23. There are gras members with stress-related effects in Arabidopsis (SCL13) 24, Rice (CIGR1 and CIGR2) 25, Tobacco (Gras1) 26, and,Poplar (SCL7) 27, FOXTAIL MILLET28, Cucumber29Hyderabad Wealth Management. Researches ConDuctal to Investigate The Impact of Abiotic Stress on the Della Gene (Gibberllic Acids ( GA), and Grain Development in SORGHUM BICOLOR (L.) Moench30. Similaly, Studies on CucumberHave Shown that certain subfamilies such as shr, scr, and della have a strong impact on the control of this spiecies29. On the Other Hand, when studying casava, S CIENTISTS ANALYSED How A Specific Gene Called Megras Responds to Various Abiotic Stresses, SUCH AS DRUUGHT, SALINITY, COLD Stress, and Exposure to Hydrogen Peroxide (H2O2) 31Udabur Investment. The Findings of the STDIES Strongly Suggest that this genes point biology31.
Our Study Invold An Extensive Genome-Wide Study of 177 MEmbers of the Wheat Gras Gene Family. Identification of these Gras generals achieved Through A Multi. Faceted Approach Including Their Classification Characterization, Expression Profiling, and Molecular Dynamic Simulations. To Confirm the Expression Pattern ObT ained fromIn-Silico Analysis, We Performed A Quantitative Real-Time Polymerase Reaction. The Findings Strongly Sugges27 is a Promising Candidate For Cing DRUGHT, Heat, and Salt Tolerance Through Genome-Editing Technology.Systematic Elucidating of the Functional ROLES Played by the Tagras Family Genes in WHEAT.
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